Neodeightonia rattanicola

Neodeightonia rattanicola

Konta & K.D. Hyde (2016)

Classification:Ascomycota-Pezizomycotina-Dothideomycetes-Dothideomycetes incertae sedis-Botryosphaeriales-Botryosphaeriaceae-Neodeightonia

Host species: Calamus sp. (Konta et al. 2016)

Holotype: MFLU 15-0294 (Konta et al. 2016)

Known distribution: Phang-Nga, Thailand

IF Number: IF552169      Mycobank Number: 552169     FOF Number: FoF02238

Description

Saprobic on rachis of Calamus sp., Sexual morph: Ascomata 180–215 high × 146–168 μm diam. (x̅ = 197 × 155 μm, n = 10), immersed to semi-immersed, solitary, scattered, uniloculate, subglobose to irregular, dark brown or black, with a long neck, irregular at the base. Ostiole central non-papillate. Peridium 35–51 μm diam. (x̅ = 45 μm, n = 10), comprising several layers, outer layer thick, comprising dark brown to black-walled cells of textura angularis, inner layer thin, comprising hyaline cells of textura angularis. Hamathecium comprising hypha-like, hyaline, septate, cellular pseudoparaphyses, up to 3.27 µm wide, often constricted at the septa. Asci 91–108 × 19–25 μm (x̅ = 101 × 22 μm, n = 10), 8-spored, bitunicate, fissitunicate, cylindrical-clavate, with thick endotunica, long pedicellate, apically thickened, with a distinct, ocular chamber. Ascospores 22–26 × 8–11 μm (x̅ = 23× 10 μm, n = 20), overlapping biseriate or obliquely 2–3-seriate, hyaline, ellipsoidal-fusiform, aseptate, with polar apiculi, smooth-walled, surrounded by thin mucilaginous sheath. Asexual morph: Coelomycetous. Conidiomata stromatic 420–510 high × 357–443 μm diam. (x̅ = 446 × 381 μm, n = 5), pycnidial, superficial, dark brown to black, covered with dense mycelium, on PDA mostly uniloculate, individual or aggregated. Paraphyses cylindrical, aseptate hyaline. Conidiogenous cell 4.4−12.5 × 1.5−4.3 μm (x̅ = 9.4 × 3.4 μm, n = 10), holoblastic, cylindrical to subcylindrical, hyaline. Conidia 13–20 × 7–8 μm (x̅ = 17.3 × 8 μm, n = 20), initially hyaline, pale to dark brown when mature.

Culture conditions: Ascospores germinating on MEA within 24 hours and germ tube produced from cell. Colonies on MEA fast growing, after 2 weeks reaching 8–9 cm diam. at 25°C, white at the edge, grey in the middle and outwardly strongly radiating. After 1 month of incubation, the colonies on MEA, grey-olivaceous and spongy, hyphae, septate, branched and smooth, form asexual morph after 6 weeks.