Neodeightonia rattanica

Neodeightonia rattanica

Konta & K.D. Hyde (2016)

Classification:Ascomycota-Pezizomycotina-Dothideomycetes-Dothideomycetes incertae sedis-Botryosphaeriales-Botryosphaeriaceae-Neodeightonia

Host species: Calamus sp. (Konta et al. 2016)

Holotype: MFLU 15-1443 (Konta et al. 2016)

Known distribution: Phang-Nga, Thailand

IF Number: IF552168      Mycobank Number: 552168     FOF Number: FoF02237

Description

Saprobic on rachis of Calamus sp. Sexual morph: Ascomata 222–241 high × 246–278 μm diam. (x̅ = 262 × 235 μm, n = 10), immersed, solitary, scattered, uniloculate, subglobose, brown to reddish brown, with a long neck, rounded at the base. Ostiole central non-papillate. Peridium 49–76 μm diam. (x̅ = 72 μm, n = 10), relatively thick, comprising several layers, outer layer thick, comprising reddish brown-walled cells of textura angularis, inner layer thin, comprising hyaline cells of textura angularis. Hamathecium comprising hypha-like, hyaline, septate, cellular pseudoparaphyses, up to 2.7 µm wide, often constricted at the septa. Asci 113–141 × 19–25 μm (x̅ = 138 × 22 μm, n = 10), 8-spored, bitunicate, fissitunicate, cylindrical-clavate, with a thick endotunica, long pedicellate, apically thickened, with a distinct, ocular chamber. Ascospores 22–25 × 8–11 μm (x̅ = 23 × 10 μm, n = 20), overlapping biseriate or obliquely biseriate, hyaline, ellipsoidal-fusiform, aseptate, often with a large guttule at the centre when immature, becoming granulate, with terminal apiculi, smooth-walled, surrounded by thick mucilaginous sheath. Asexual morph: Coelomycetous. Conidiomata stromatic 245–349 high × 208–305 μm diam. (x̅ = 297 × 255 μm, n = 5), pycnidial, superficial, purplish to black, covered with dense mycelium, on PDA uni-to multilocular, individual or aggregated. Paraphyses cylindrical, aseptate, hyaline. Conidiogenous cells 6−11 × 2−3 μm (x̅ = 9.7 × 2.7 μm, n = 10), holoblastic, cylindrical to subcylindrical, hyaline. Conidia 19–22 × 7–9 μm (x̅ = 20.7 × 8.4 μm, n = 20), initially hyaline, pale to dark brown when mature, unicellular, ellipsoid to obovoid, thick-walled, with granular content, rounded at the apex.

Culture conditions: Ascospores germinating on MEA within 24 hours and germ tubes produced from both ends. Colonies on MEA fast growing, after 2 weeks reaching 7–8.5 cm diam. at 25 °C, white at the edge, grey in the middle, outwardly strongly radiating. After 5 months of incubation, the colonies on MEA, becoming grey-olivaceous and spongy, hyphae, septate, branched and smooth, form asexual morph after 2 months.